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A bacterial strain RMLRT03 with ability to decolorize textile dye Acid Orange dye was isolated from textile effluent contaminated soil of Tanda, Ambedkar Nagar, Uttar Pradesh (India). The decolorization studies were performed in Bushnell and Haas medium (BHM) amended with Acid Orange dye. The bacterial strain was identified as Staphylococcus hominis on the basis of 16S rDNA sequence. The bacterial strain exhibited good decolorization ability with glucose and yeast extract supplementation as cosubstrate in static conditions. The optimal condition for the decolorization of Acid Orange dye by Staphylococcus hominis RMLRT03 strain were at pH 7.0 and 35oC in 60 h of incubation. The bacterial strain could tolerate high concentrations of Acid Orange dye up to 600 mg l‑1. The high decolorizing activity under natural environmental conditions indicates that the bacterial strain has practical application in the treatment of dye containing wastewaters.

Keywords

16S rDNA, BHM, cosubstrate, decolorization, textile dye

Full Text

Efficient Decolorization of Dye Acid Blue 113 by Soil Bacterium Bacillus subtilis RMLP2

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Authors

Pradeep Kumar Singh ¹, Rajat Pratap Singh ², Pankaj Singh ³, Ram Lakhan Singh ¹

Affiliations
1 Department of Biochemistry, Dr. Rammanohar Lohia Avadh University, Ayodhya – 224001, Uttar Pradesh, IN
2 Department of Biotechnology, Guru Ghasidas University, Koni, Bilaspur – 495009, Chhattisgarh, IN
3 Department of Biotechnology, Dr. Rammanohar Lohia Avadh University, Ayodhya - 224001, Uttar Pradesh, IN

Source

Toxicology International (Formerly Indian Journal of Toxicology), Vol 28, No 3 (2021), Pagination: 267-278

Abstract

In this study, a bacterial strain was isolated from soil and tested for the decolorization of azo dye Acid Blue 113 (AB 113). Decolorization of azo dyes by means of physico-chemical method is not environmentally friendly thus an alternative method based on bacteria was employed for decolorization of AB 113. The color removal studies were performed using Bushnell and Hass medium amended with AB 113 dye. Bacterial isolate Bacillus subtilis RMLP2 was identified by 16S rRNA gene sequence analysis. The effect of various physico-chemical parameters such as incubation condition, pH, temperature, carbon source, nitrogen source and dye concentration on decolorization of AB 113 by Bacillus subtilis RMLP2 were studied. The bacterial isolate showed the remarkable higher percentage (92.71%) of color removal of dye AB 113 at 100 mg/L concentration, 35^oC, pH 7 during 72 h of incubation period under static condition. Yeast extract and glucose was found as best nitrogen and carbon source for efficient decolorization of dye. These results confirmed that the Bacillus subtilis has enormous ability to degrade dye AB 113 present in textile effluents.

Keywords

Acid Blue 113, Azo Dye, Bacillus subtilis, Decolorization, 16S rDNA.

Full Text

References

Chacko JT, Subramaniam K. Enzymatic degradation of azo dyes - A review. Int J Environ Sci. 2011; 1(6):1250–60.

Singh RP, Singh PK, Singh RL. Present status of biodegradation of textile dyes. Curr Trends Biomed Eng Biosci. 2017; 3(4):555618. https://doi.org/10.19080/CTBEB.2017.03.555618

Shah M. Biodegradation of azo dyes by three isolated bacterial strains: An environmental bioremedial approach. J Microbial Biochem Technol. 2014; S3(007):1–5. https://doi.org/10.4172/1948-5948.S3-007

John J, Dineshram R, Hemalatha KR, Dhassiah MP, Gopal D, Kumar A. Bio decolorization of synthetic dyes by a halophilic bacterium Salinivibrio sp. Front Microbiol. 2020; 11:594011. https://doi.org/10.3389/fmicb.2020.594011

Singh RP, Singh PK, Singh RL. Bacterial decolorization of textile azo dye Acid Orange by Staphylococcus hominis RMLRT03. Toxicol Int. 2014; 21:160–6. https://doi.org/10.4103/0971-6580.139797

Chang JS, Chou C, Lin YC, Lin PJ, Ho JY, Hu TL. Kinetic characteristics of bacterial azo dye decolorization by Pseudomonas luteola. Water Research 2001; 35(12):2841–50. https://doi.org/10.1016/S0043-1354(0 0)00581-9

Zin KM, Halmi MIE, Gani SSA, Zaidan UH, Samsuri AW, Shukor MYA. Microbial decolorization of triazo dye, Direct Blue 71: An optimization approach using Response Surface Methodology (RSM) and Artificial Neural Network (ANN). BioMed Res Int. 2020; Article ID 2734135. https://doi.org/10.1155/2020/2734135

Maas R, Chaudhari S. Adsorption and biological decolorization of azo dye Reactive Red 2 in semicontinuous anaerobic reactors. Process Biochem. 2005; 40:699-5. https://doi.org/10.1016/j.procbio.2004.01.038

Voulvoulis N, Georges K. Industrial and agricultural sources and pathways of aquatic pollution. In: Bugyi G, McKeown AE, eds. Impact of water pollution on human health and environmental sustainability. IGI Global, Hershey and nbsp. 2015. https://doi.org/10.4018/978-1-4666-9559-7.ch002

Ogunlaja A, Nwankwo IN, Omaliko ME, Olukanni OD. Biodegradation of Methylene Blue as an evidence of synthetic dyes mineralization during textile effluent biotreatment by Acinetobacter pittii. Environ Process. 2020; 7:931–47. https://doi.org/10.1007/s40710-020- 00443-6

Lade HS, Waghmode TR, Kadam AA, Govindwar SP. Enhanced biodegradation and detoxification of disperse azo dye Rubine GFL and textile industry effluent by defined fungal-bacterial consortium. Biodeter Biodegrad. 2012; 72:94–107. https://doi.org/10.1016/j.ibiod.2012.06.001

Singh RL, Singh PK, Singh RP. Recent advances in decolorization and degradation of dyes in textile effluent by biological approaches. Boca Raton, New York: CRC Press; 2020; https://doi.org/10.1201/9780429244322

Das A, Mishra S. Decolorization of different textile azo dyes using an isolated bacterium Enterococcus durans GM13. Int J Curr Microbiol App Sci. 2016; 5(7):675– 86. https://doi.org/10.20546/ijcmas.2016.507.077

Nama S, Rao DM, Devanna N. Decolorization of Alizarin Red S dye by bacterial strains isolated from industrial effluents. Int J Plant Anim Environ Sci. 2016; 6(1):268–75.

Yaseen DA, Scholz M. Treatment of synthetic textile wastewater containing dye mixtures with microcosms. Environ Sci Pollut Res. 2018; 25:1980–97. https://doi.org/10.1007/s11356-017-0633-7

Aktar K, Zerin T, Banik A. Biodegradation of textile dyes by bacteria isolated from textile industry effluents. Stamford J Microbiol. 2019; 9(1):5–8. https://doi.org/10.3329/sjm.v9i1.45649

Mountassir Y, Benyaich A, Rezrazi M, Bercot P, Gebrati L. Wastewater effluent characteristics from Moroccan textile industry. Water Sci Technol. 2013; 67:2791–9. https://doi.org/10.2166/wst.2013.205

Olukanni OD, Osuntoki A, Gbenle GO. Decolorization of azo dyes by strain of Micrococcus isolated from a reuse dump soil. J Biotechnol. 2009; 8:442–8. https://doi.org/10.3923/biotech.2009.442.448

Burakov AE, Galunin EV, Burakova IV, Kucherova AE, Agarwal S, Tkachev AG, Gupta VK. Adsorption of heavy metals on conventional and nanostructured materials for wastewater treatment purposes: a review. Ecotoxicol Environ Saf. 2018; 148:702–12. https://doi.org/10.1016/j.ecoenv.2017.11.034

Mohanty SS, Kumar A. Enhanced degradation of anthraquinone dyes by microbial monoculture and developed consortium through the production of specific enzymes. Sci Rep. 2021; 11:7678. https://doi.org/10.1038/s41598-021-87227-6

Radwan EK, Kafafy H, El-Wakeel ST, Shaheen TI, Gad- Allah TA, El-Kallin AS, El-Naggar ME. Remediation of Cd (II) and Reactive Red 195 dye in wastewater by nanosized gels of grafted carboxymethyl cellulose. Cellulose 2018; 25:6645–60. https://doi.org/10.1007/s10570-018-2003-0

Asfaram A, Ghaedi M, Agarwal S, Tyagi I, Gupta VK. Removal of basic dye Auramine-O by ZnS:Cu nanoparticles loaded on activated carbon: optimization of parameters using response surface methodology with central composite design. RSC Adv. 2015; 5:18438–50. https://doi.org/10.1039/C4RA15637D

Ugwuja C, Adelowo O, Ogunlaja A, Omorogie MO, Olukanni OD, Ikhimiukor OO, Iermak I, Kolawole GA, Guenter C, Taubert A, Bodede O, Moodley R, Inada NM, de Camargo ASS, Unuabonah EI. Visible light mediated photodynamic water disinfection @ bimetallic doped hybrid clay nanocomposites. ACS Appl Mater Interfaces. 2019; 11:25483–94. https://doi.org/10.1021/acsami.9b01212

Singh RL, Singh PK Singh RP. Enzymatic decolorization and degradation of azo dyes-a review. Int Biodeter Biodegr. 2015; 104:21–31. https://doi.org/10.1016/j.ibiod.2015.04.027

Olukanni OD, Osuntoki AA, Keyalni DC, Gbenle GO, Govindwar SP. Decolorization and biodegradation of Reactive Blue 13 by Proteus mirabilis LAG. J Hazard Mater. 2010; 184:290–8. https://doi.org/10.1016/j.jhazmat.2010.08.035

Zablocka-Godlewska E, Przystas W, Grabinska-Sota E. Possibilities of obtaining from highly polluted environments: New bacterial strains with a significant decolorization potential of different synthetic dyes. Water Air Soil Pollut. 2018; 229:176. https://doi.org/10.1007/s11270-018-3829-7

Solis M, Solis A, Perez HI, Manjarrez N, Flores M. Microbial decolorization of azo dyes: A Review. Process Biochem. 2012; 47:1723–48. https://doi.org/10.1016/j.procbio.2012.08.014

Thangaraj S, Jooju B, Sadasivam SK. Decolourization of textile azo dyes by a newly isolated bacterial strain. Appl Ecol Environ Sci. 2021; 9(2):203–8. https://doi.org/10.12691/aees-9-2-12

Singh RN, Kaushik R, Arora DK, Saxena AK. Prevalence of opportunist pathogens in thermal springs of devotion. J Appl Sci Environ Sanit. 2013; 8:195–203.

Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular Evolutionary Genetics analysis. Version 6.0. Mol Biol Evol. 2013; 30:2725–9. https:// doi.org/10.1093/molbev/mst197

Bayoumi MN, Al-Wasify RS, Hamed SR. Bioremediation of textile wastewater dyes using local bacterial isolates. Int J Curr Microbiol Appl Sci. 2014; 3:962–70.

Kalyani DC, Telke AA, Dhanve RS, Jadhav JP. Ecofriendly biodegradation and detoxification of Reactive Red 2 textile dye by newly isolated Pseudomonas sp. SUK1. J Hazard Mater. 2009; 163:735–42. https://doi.org/10.1016/j.jhazmat.2008.07.020

Aravindhan R, Rao JR, Nair BU. Removal of basic yellow dye from aqueous solution by sorption on green alga Caulerpa scalpelliformis. J Hazard Mater. 2007; 142:68–76. https://doi.org/10.1016/j.jhazmat.2006.07.058

Kumar K, Devi SS, Krishnamurthi K, Dutta D, Chakrabarti T. Decolorization and detoxification of Direct Blue 15 by a bacterial consortium. Bioresour Technol. 2007; 98:3168–71. https://doi.org/10.1016/j.biortech.2006.10.037

Patil P, Phugare S, Jadhav S, Jadhav J. Communal action of microbial cultures for Red HE3B degradation. J Hazard Mater. 2010; 181(1-3):263–70. https://doi.org/10.1016/j.jhazmat.2010.05.006

Unnikrishnan S, Khan MH, Ramalingam K. Dye tolerant marine Acinetobacter baumannii mediated biodegradation of Reactive Red. Water Sci Eng. 2018; 11:265–75. https://doi.org/10.1016/j.wse.2018.08.001

Hashem RA, Samir R, Essam TM, Ali EA, Amin MA. Optimization and enhancement of textile Reactive Remazol Black B decolorization and detoxification by environmentally isolated pH tolerant Pseudomonas aeruginosa KY284155. AMB Expr. 2018; 8:83 https://doi.org/10.1186/s13568-018-0616-1

Chang JS, Lin YC. Fed-batch bioreactor strategies for microbial decolorization of azo dye using a Pseudomonas luteola strain. Biotechnol Prog. 2000; 16:979–85. https://doi.org/10.1021/bp000116z

Shah MP, Pate KA. Optimization of environmental parameters on microbial degradation of Reactive Black Dye. J Bioremed Biodeg. 2013; 4(3). https://doi.org/10.4172/2155-6199.1000183

Arulazhagan P. A study on microbial decolorization of Reactive Red M8B by Bacillus subtilis isolated from dye contaminated soil samples. Int J Curr Res Biol Med. 2016; 1:1–13.

Joe MH, Lim SY, Kim DH, Lee IS. Decolorization of reactive dyes by Clostridium bifermentans SL186 isolated from contaminated soil. World J Microbiol Biotechnol. 2008; 221–6. https://doi.org/10.1007/s11274-008-9733-3

Carolin CF, Kumar PS, Joshiba GJ. Sustainable approach to decolorize Methyl Orange dye from aqueous solution using novel bacterial strain and its metabolites characterization. Clean Technol Envir. 2020. https://doi.org/10.1007/s10098-020-01934-8

Gonzalez GLV, Gonzalez AG, Escamilla SEM. Proposed pathways for the reduction of a reactive azo dye in an anaerobic fixed bed reactor. World J Microbiol Biotechnol. 2009; 25:415–26. https://doi.org/10.1007/s11274-008-9906-0

Telke A, Kalyani D, Jadhav J, Govindwar S. Kinetics and mechanism of Reactive Red 141 degradation by a bacterial isolate Rhizobium radiobacter MTCC 8161. Acta Chim Slov. 2008; 55:320–9.

Saratale RG, Saratale GD, Kalayani DC, Chang JS, Govindwar SP. Enhanced decolorization and biodegradation of textile azo dye Scarlet R by using developed microbial consortium-GR. Bioresour Technol. 2009; 100:2493–500. https://doi.org/10.1016/j.biortech.2008.12.013

Roy DC, Biswas SK, Saha AK, Sikdar B, Rahman M, Roy AK, Prodhan ZH, Tang SS. Biodegradation of Crystal Violet dye by bacteria isolated from textile industry effluents. Peer J. 2018; 21(6):e5015. https://doi.org/10.7717/peerj.5015

Fan L, Zhu SN, Liu DQ, Ni JR. Decolorization of 1-amino-4-bromoanthraquinone-2-sulfonic acid by a newly isolated strain of Sphingomonas herbicidovorans. Int Biodeter Biodegr. 2009; 63:88–92. https://doi.org/10.1016/j.ibiod.2008.07.004

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